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Figure 2 | BMC Plant Biology

Figure 2

From: Development and characterization of an oat TILLING-population and identification of mutations in lignin and β-glucan biosynthesis genes

Figure 2

Schematics of TILLING population development. Figure shows the schematics for the step-wise preparation of the TILLING library. Upon EMS treatment, M1 seeds were sown in soil and M1 generation plants allowed to self-pollinate. M2 seeds from individual line were collected separately and one seed per line sown in soil. Once germinated, leaf samples were collected for DNA preparation and the plants allowed to set seeds. M3 seeds were then collected and stored. Preparations are underway to setup the seed database at Nordgen.

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