| Stage of plantlet micropropagation | ||||
---|---|---|---|---|---|
Plant growth regulators in basal medium (BM) | PLS germination | Embryogenic callus induction on callus induction medium | Embryogenic/organogenic callus culture on callus maintenance medium (A4 medium) | Shoot differentiation, PLB multiplication and elongation on A10 medium | PLB - derived plantlet rooting on A5 medium |
Thidiazuron (TDZ) | 0.5 mg l-1 | 0.5 mg l-1 | 0.3 mg l-1 | 0 | 0 |
Indole-3-acetic acid (IAA) | 0 | 0.5 mg l-1 | 0.5 mg l-1 | 0 | 0 |
α-Naphthalene acetic acid (NAA) | 0 | 0 | 0 | 0.5 mg l-1 | 0 |
Phytagel | 7.5 g l-1 | 7.5 g l-1 | 7.5 g l-1 | 7.5 g l-1 | 7.5 g l-1 |
Other features | Â | Â | Â | Â | Â |
Container | Petri disk | Petri disk | tube | Flask | tube |
Medium volume | 20 ml | 20 ml | 20 ml | 100 ml | 20 ml |
Subcultures | No | No | Every 21 days | No | No |
Culture time | 4 - 6 months | 2 months | 6 months | 4 months | 2 months |