Table 2 Description of tissues
tissue ID | cell line | culture | medium | developmental stage | embryogeneity | ploidy |
|---|---|---|---|---|---|---|
1.2 | F1 out of 3-2-0503 | zygotic embryo | in planta | 84-85 d after pollination | yes | diploid |
1.3 | F1 out of 3-2-0503 | zygotic embryo | in planta | 99-101 d after pollination | yes | diploid |
2.1.1 | 3-14-0805 | callus | standard | 0 h before transfer to standard media without hormones | yes, but precocious germination | diploid |
2.1.4 | 3-43-0503 | callus | standard | 0 h before transfer to standard media without hormones | no longer | tetraploid |
2.1.6 | 3-43-0503 | callus | standard without growth regulators | 0 h before transfer to standard media without hormones | no longer | tetraploid |
2.1.6B | 3-45-0503 | callus | standard without growth regulators | 0 h before transfer to standard media without hormones | no longer | tetraploid |
2.1.7 | 3-75-0503 | suspension | standard | 0 h before transfer to standard media without hormones | yes, but no torpedo-shaped s.e. | tetraploid |
2.1.9 | 3-75-0503 | suspension | standard without growth regulators | 3d after transfer to standard media without hormones | yes, but no torpedo-shaped s.e. | tetraploid |
2.1.10 | 3-76-0503 | suspension | standard | 0 h before transfer to standard media without hormones | yes, torpedo-shaped s.e. | tetraploid |
2.1.11 | 3-76-0503 | suspension | U without growth regulators | 4 h after transfer to U media without hormones | yes, torpedo-shaped s.e. | tetraploid |
2.1.12 | 3-76-0503 | suspension | U without growth regulators | 3d after transfer to U media without hormones | yes, torpedo-shaped s.e. | tetraploid |
2.1.13 | 3-76-0503 | somatic embryo out of suspension | U without growth regulators | 20-22d - globular-shaped somatic embryo | yes, torpedo-shaped s.e. | tetraploid |
2.1.14 | 3-76-0503 | somatic embryo out of suspension | U without growth regulators | 20-22d - torpedo-shaped somatic embryo | yes, torpedo-shaped s.e. | tetraploid |
2.1.15 | 3-76-0503 | somatic embryo out of suspension | U without growth regulators | 20-22d - torpedo-shaped somatic embryo, precocious root germination | yes, torpedo-shaped s.e. | tetraploid |
2.1.17 | 3-76-0503 | somatic embryo out of suspension | U without growth regulators | 20-22d - torpedo-shaped somatic embryo (following two step desiccation: 4d 97% and 3d 92% relative humidity) | yes, torpedo-shaped s.e. | tetraploid |
2.3.1 | 3738-12G | callus | standard | 0 h before transfer to standard media without hormones | never | diploid |
2.3.3 | 3738-12G | callus | standard without growth regulators | 3d after transfer to standard media without hormones | never | diploid |