Figure 3
From: Tomato root transcriptome response to a nitrogen-enriched soil patch
qRT-PCR analysis of key nitrogen metabolism genes. Expression levels of NH4+ transporters AMT1 and AMT2, glutamine synthetases GTS1 and GS, NO3- transporter NRT2.1, nitrite reductase Nii, NADH-dependant glutamate synthase GLT1 (GOGAT), glutamate dehydrogenase GDH1, ferrodoxin-dependant glutamate synthase GLS1.2 (GOGAT), and asparagine synthetase ASN1 in patch roots harvested 53 hrs after high (65 μg NH4+-N g-1 soil), low (6.5 μg NH4+-N g-1 soil), or water control treatments. Relative quantity was calculated using the ΔΔCT method with actin (LeACT) as the reference control, and the water control group normalized to 1. For a given gene, means followed by different letters are significantly different from one another at P < 0.05 (one way ANOVA).