Phenotypes and genetic constitution of seeds used for transcriptional profiling. (A-G) Confocal micrographs of seeds at 5 days after pollination (7 days after flowering for msi1). Boxes surround the chalazal region. Below each composite seed image is an enlargement showing peripheral endosperm. Seed genotypes are: (A) 6xX2x, (B) 4xX2x, (C) 2xX2x, (D) 2xX4x, (E) 2xX6x, (F) fis1X2x, (G) msi1 (autonomous seed produced by an unfertilized msi1mutant ovule). Arrow in (F) indicates endosperm nodules. (H) Spectrum of maternal:paternal genomic balance. Above bar: ratio of maternally to paternally derived genomes in the endosperm for each 'cross' (sexual crosses + unfertilized msi1). Below bar: absolute number of genomes in endosperm (EN) and embryo (EM) for each 'cross'.