Figure 2

ProDH2 expression complements a yeast Δ put1 mutant. Yeast wildtype or Δput1 mutant with various ProDH expression constructs were grown for 4 d on minimal medium with 2% (w/v) galactose (Gal) as inducing carbon source and 10 mM Pro (upper plate) or 10 mM urea (lower plate) as the sole nitrogen source. Expression of ScPut1 confers wildtype growth to the Δput1 mutant. Native AtProDH pre-proteins do not complement the Pro utilisation deficiency of the Δput1 mutant. Replacement of the predicted Arabidopsis mTPs by the mTP of the yeast Sdh1 gene confers functional expression and the capability to metabolise external Pro as N-source (mTP-AtProDH1 and mTP-AtProDH2).