Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour

Table 1 Best matches of consensus sequences from Butte 86 contigs to NCBI non-redundant database.

Contig #	# ESTs	NCBI Accession¹	Identity	Source	Type
1	19²	[GenBank: AF234644.1]	1195/1227	T. aestivum cv. Cheyenne	mRNA
2	34	[GenBank: M16064.1]	1187/1200	T. aestivum cv. Yamhill	gene
3	18³	[GenBank: M36999.1]	1088/1094	T. aestivum cv. Yamhill	gene
4	14	[GenBank: FJ006605.1]	1005/1007	T. aestivum cv. Chinese Spring	gene
5	27	[GenBank: FJ006695.1]	1139/1191	Aegilops speltoides	gene
6	17	[GenBank: EF426565.1]	1048/1054	T. turgidum ssp. durum cv. Langdon	gene
7	4	[GenBank: M13713.1]	1083/1087	T. aestivum cv. Yamhill	gene
8	8²	[GenBank: AF234644.1]	1083/1123	T. aestivum cv. Cheyenne	mRNA
9⁴	3	[GenBank: FJ006688.1]	848/908	Aegilops searsii	gene
		[GenBank: FJ006686.1]	848/908	Aegilops searsii	gene
10	10³	[GenBank: M36999.1]	1024/1078	T. aestivum cv. Yamhill	gene
11⁵	3	[GenBank: EF151018.1]	668/668	T. aestivum cv. Chinese Spring	mRNA

¹ with best score by blastn against nr/nt database, no filters, no masks, last searched on 5/11/09.
² includes seven ESTs assigned to both contigs #1 and #8.
³ includes nine ESTs assigned to both contigs #3 and 10.
⁴ contig is missing a portion of the 3' end of coding region.
⁵ contig is missing a portion of the 5' end of the coding region.

ISSN: 1471-2229