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Table 1 Oligonucleotides used in PCR and quantitative RT-PCR

From: Genetic transformation of cotton with a harpin-encoding gene hpa Xoo confers an enhanced defense response against different pathogens through a priming mechanism

Gene Primer sequence Anneal temperature (°C) Segment length (bp)
hpa1Xoo(EF028092*) forward:5'-TTCGGATCCATGAACTCTTTGAACACACAATT-3'
reverse:5'-GGTGAGCTCTTACTGCATCGATGCGCT-3'
56 438
35S forward:5'-AGAGGCTTACGCAGCAGGTC-3'
reverse:5'-GCCAGTCTTTACGGCGAGTT-3'
52 310
NOS forward:5'-GAACTGACAGAACCGCAACG-3'
reverse:5'-ACCGAGGGGAATTTATGGAA-3'
50 180
GhAOX 1(DQ250028) forward:5'-GCGCCTGGGGATGATGATGAGTCGTG-3'
reverse:5'-GCGCTTCAGTGATAACCGAGCGGAG-3'
57 1298
hsr203J(X77136) forward:5'-TGTACTACACTGTCTACACGC-3'
reverse:5'-GATAAAAGCTATGTCCCACTCC-3'
55 618
EF-1α (AJ223969) forward:5'-AGACCACCAAGTACTACTGCAC-3'
reverse:5'-CCACCAATCTTGTACACATCC-3'
58 495
Ghdhg-OMT(GQ303569) forward:5'-ATGAATATGGGCAATGCTAAT-3'
reverse:5'-TCAGGGGTAAACCTCAATGAGA-3'
53 1087
npr1(U76707) forword:5'-GGCCTCGAGATGGCTTATTTGTCTGAGCCATCATCT-3'
reverse:5'-CGTCTCGAGTCACAATTTCCTATACTTGTAGG-3'
62 1794
hin1(Y07563) forword:5'-GAACGGAGCCTATTATGGCCCTTCC-3'
reverse:5'-CATGTATATCAATGAACACTAAACGCCGG-3'
55 867
  1. * GenBank accession numbers