Skip to main content

Table 1 Oligonucleotides used in PCR and quantitative RT-PCR

From: Genetic transformation of cotton with a harpin-encoding gene hpa Xoo confers an enhanced defense response against different pathogens through a priming mechanism

Gene

Primer sequence

Anneal temperature (°C)

Segment length (bp)

hpa1Xoo(EF028092*)

forward:5'-TTCGGATCCATGAACTCTTTGAACACACAATT-3'

reverse:5'-GGTGAGCTCTTACTGCATCGATGCGCT-3'

56

438

35S

forward:5'-AGAGGCTTACGCAGCAGGTC-3'

reverse:5'-GCCAGTCTTTACGGCGAGTT-3'

52

310

NOS

forward:5'-GAACTGACAGAACCGCAACG-3'

reverse:5'-ACCGAGGGGAATTTATGGAA-3'

50

180

GhAOX 1(DQ250028)

forward:5'-GCGCCTGGGGATGATGATGAGTCGTG-3'

reverse:5'-GCGCTTCAGTGATAACCGAGCGGAG-3'

57

1298

hsr203J(X77136)

forward:5'-TGTACTACACTGTCTACACGC-3'

reverse:5'-GATAAAAGCTATGTCCCACTCC-3'

55

618

EF-1α (AJ223969)

forward:5'-AGACCACCAAGTACTACTGCAC-3'

reverse:5'-CCACCAATCTTGTACACATCC-3'

58

495

Ghdhg-OMT(GQ303569)

forward:5'-ATGAATATGGGCAATGCTAAT-3'

reverse:5'-TCAGGGGTAAACCTCAATGAGA-3'

53

1087

npr1(U76707)

forword:5'-GGCCTCGAGATGGCTTATTTGTCTGAGCCATCATCT-3'

reverse:5'-CGTCTCGAGTCACAATTTCCTATACTTGTAGG-3'

62

1794

hin1(Y07563)

forword:5'-GAACGGAGCCTATTATGGCCCTTCC-3'

reverse:5'-CATGTATATCAATGAACACTAAACGCCGG-3'

55

867

  1. * GenBank accession numbers