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Figure 6 | BMC Plant Biology

Figure 6

From: Genetic transformation of cotton with a harpin-encoding gene hpa Xoo confers an enhanced defense response against different pathogens through a priming mechanism

Figure 6

Viability of cotton cells in the presence of conidia of Verticillium dahliae. (a) (1) Living (red arrow) or dead (white arrow) cells in the cell suspension of untransformed Z35 mixed with conidia of V. dahliae under a conventional light microscope. (2) Living (red arrow) or dead (white arrow) cells in the cell suspension of hpa1 Xoo -transformed T-34 mixed with conidia of V. dahliae under a conventional light microscope. (3) Fluorescence emitted from living cells (red arrow) of untransformed Z35 mixed with conidia of V. dahliae under a fluorescence microscope. (4) Fluorescence emitted from living cells (red arrow) of hpa1 Xoo -transformed T-34 mixed with conidia of V. dahliae under a fluorescence microscope. (1), (2), (3), and (4) scale bars = 300 μm. (b) Percentage of the cell death in the cotton cell suspension mixed with V. dahliae conidia. (c) Percentage of cotton cells in the absence of conidia of Verticillium dahliae. Cotton cells and V. dahliae conidia were mixed in a ratio of 1:20. The percentage of cell death was counted at 3, 6, 9, and 12 h after mixing. Error bars indicate standard error of the mean (n = 3). Data points marked with asterisks are significantly different (Student's t test, p < 0.01). The experiment was repeated three times.

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