Figure 4
Generation of active oxygen species (AOS) in leaves of hpa1 Xoo -transformed T-34 and untransformed Z35. (a) Oxygen burst in cotton leaves dipped in the conidial suspension of Verticillium dahliae collected from 0 to 3 hr after inoculation (red arrow points to the location of oxygen burst). (b) Light microscopy of the oxygen burst in leaves of untransformed Z35 (1) and hpa1 Xoo -transformed T-34 (2) 3 h after the inoculation. (c) H2O2 content (μg/g fresh weight) in leaves of hpa1 Xoo -transformed T-34 and untransformed Z35 dipped in the conidial suspension of V. dahliae (mean values and standard errors calculated from three replicates). 1, non-inoculated; 2, inoculated. (d) Quantitative RT-PCR analysis of ghAOX1, hin1, npr1, ghdhg-OMT, and hsr203J expression in leaves of hpa1 Xoo -transformed T-34 (T-34-i) and untransformed Z35 (Z35-i) dipped in the conidial suspension of V. dahliae compared with that of hpa1 Xoo -transformed T-34 (T-34) and untransformed Z35 (Z35) dipped in water (error bars indicate standard error). b (1, 2) scale bars = 0.01 mm. The experiment was repeated three times. Asterisks represent significant differences at the level of 0.01.