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Figure 3 | BMC Plant Biology

Figure 3

From: Genetic transformation of cotton with a harpin-encoding gene hpa Xoo confers an enhanced defense response against different pathogens through a priming mechanism

Figure 3

Immuno-gold localization of harpin Xoo in leaves and stem apices of hpa1 Xoo -transformed T-34 and untransformed Z35. (a) and (b) Stem apices of hpa1 Xoo -transformed T-34. (c) Stem apices of untransformed Z35. (d) and (e) Leaves of hpa1 Xoo -transformed T-34. (f) Leaves of untransformed Z35. CW: cell wall, Cy: cytoplasm, V: vacuole, IS: intercellular space, Ch: chloroplasts, M: mitochondria. Bars: a and d = 5 μm; b, c, e, and f = 0.2 μm. Arrow points to gold particles labeled with harpinXoo antiserum (15 nm particles). The squares indicate the regions of b and e magnified in a and d, respectively. More than 20 ultrathin sections of each sample were examined with a JEM × 1200 transmission electron microscope (Nikon, Japan). The experiment was repeated twice.

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BMC Plant Biology

ISSN: 1471-2229

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