Figure 1

Molecular analysis of hpa1 _Xoo -transformed T-34 and untransformed cotton. (Gossypium hirsutum)Z35. (a) The schematic representation of recombinant plasmid pBI35S-hpa1 _Xoo -nptII. R and L represent the right and left borders of T-DNA. (b) Amplifications of hpa1 _Xoo (1), 35S promoter (2), and NOS terminator (3) in PCR; hpa1 _Xoo (h), 35S promoter (35S), and NOS terminator (NOS) represented the DNA fragment amplified from the positive control. M: marker; Four individual plants (1, 2, 3, 4) in T6 progeny of T-34 were tested. (c) Southern blot analysis of hrp1 _Xoo insertions in T-34 and Z35. Ten micrograms of genomic DNA was digested with EcoRI and hybridized against a DIG-labeled hpa1 _Xoo probe. M: marker. Four plants (1, 2, 3, 4) in T6 progeny of T-34 were tested. (d) Western blot analysis of harpin_Xoo in T-34 transgenic lines. Four plants (1, 2, 3, 4) in T6 progeny of T-34 were tested. Purified harpin_Xoo served as the positive control.