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Table 4 Selected genes for functional characterization by VIGS

From: SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): identification of early mediators of insect responses

Tag ID

Gene Name

VIGS construct

Silencing efficiency (%)1

Tag-6205

Nematode Resistance Protein (NRP)

pTVNRP

67 ± 1.8

Tag-10039

Lipid Phosphate Phosphatase (LPP)

pTVLPP

69 ± 2.1

Tag-11559

Wall Assocaited Kinase (WAK)

pTVWAK

83 ± 2.5

Tag-2815

Nicotiana Elicited Induced Gene (NEIG)

pTVNEIG

91 ± 5.2

Tag-837

UnkA

pTVUNKA

73 ± 4.7

Tag-12314

UnkB

pTVUNKB

98 ± 2.8

  1. 1 The silencing efficiency is expressed as the reduction (%) of the mRNA levels in the VIGS-silenced plants relative to the levels of the corresponding mRNA in EV (empty vector) plants. In all cases the reductions were statistically significant (P < 0.05, t-test, EV vs VIGS).