Figure 5

WT/ hen1-1 grafting experiments. Analysis of mature miR395, miR399 and miR171 by RNA gel blot analysis in scions and rootstocks of reciprocal hen1-1/WT and WT/hen1-1 grafts under sulfate and phosphate deficiency. A: miRNAs 395 and 399 were translocated from WT scions to hen1-1 rootstocks but not in the opposite direction, miR171 was immobile. One representative result is shown for WT, and three replications for hen1-1 roots and shoots. The hen1-1 graft parts kept their growth retardation phenotype, indicating that not all necessary miRNAs could be transferred. The 5.8 ribosomal RNA band served as a loading control. B: Control of miR395 expression in WT and hen1-1 mutant plants. In WT plants miR395 was induced by sulfate deficiency in shoots and roots, while no signal was detected in hen1-1 mutants under both conditions.