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Table 1 Results from eight TILLING assays in the B. rapa mutant population.

From: A rich TILLING resource for studying gene function in Brassica rapa

Gene name EMS (%) Length (bp) GC (%) Mutations detected Screened M2 plants Est. mutations per M2 plant* M2 plants in population Mutation density** Expected mutations kb-1...
BraA.RPL.a 0.3 1007 47 21 768 13577 (10111) 6912 1/37 (1/49) 188 (164)
BraA.RPL.b 0.3 1072 45 149 4608 15082 (11730) 6912 1/33 (1/43) 209 (190)
BraA.RPL.c 0.3 1001 47 132 4608 14309 (10656) 6912 1/35 (1/47) 198 (173)
BraA.IND.a 0.3 1004 41 35 3072 5674 (4844) 6912 1/88 (1/103) 78 (78)
BraA.MET1.a 0.3 1104 48 94 3072 13858 (10105) 6912 1/36 (1/49) 191 (164)
BraA.MET2.b 0.3 1007 47 89 3072 14385 (10712) 6912 1/35 (1/47) 199 (173)
Relevant averages       12814 (9693)   1/44 (1/56) 177 (157)
BraA.RPL.b 0.4 1072 45 57 2304 11539 (8975) 2304 1/43 (1/56) 53 (48)
BraA.RPL.c 0.4 1001 47 40 2304 8672 (6458) 2304 1/358(1/77) 40 (35)
Relevant averages       10106 (7717)   1/51 (1/67) 47 (42)
  1. * The estimated number of mutations per plant was calculated using an estimated genome size of 500 Mb for B. rapa [30]. Numbers in brackets were obtained after normalising to the average 35% GC content for the B. rapa genome [29].
  2. ** Mutation density based on estimated number of mutations per plant divided by the 500,000 kb
  3. genome. Numbers in brackets were obtained after normalising to the average 35%
  4. GC content for the B. rapa genome [29].
  5. *** The number of expected mutations per kb in the population was obtained by extrapolating the
  6. number of detected mutations in a subset of the M2 plants to the whole population.
  7. Numbers in brackets were obtained after normalising to the average 41% GC content for B. rapa
  8. exons [29].