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Table 2 Primers used in this study (Incorporated restriction site sequences are underlined)

From: Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites

Primer Sequence (5'-3')
Walker1 CTA ATA CGA CTC ACT ATA GGG NNN NA TGC
Walker2 CTA ATA CGA CTC ACT ATA GGG NNN NT AGC
Walker3 CTA ATA CGA CTC ACT ATA GGG NNN NG ATC
Walker4 CTA ATA CGA CTC ACT ATA GGG NNN NC TAG
Adaptor walker CTA ATA CGA CTC ACT ATA GGG
R1 GAT AAT GAT CAC GTC GAA TTG AG
R2 CTC TGG CTT TGA TGT GGGT GCT
D0-F CCC AAGCTT GAC TTC ACC CTT CTT TCT TGT C
D1-F CCC AAGCTT GAT CTA CTG GAA ATT GAC CAA C
D2-F CCC AAGCTT TCA CTA TGT TTG TTA GTG CTT G
D3-F CCC AAGCTT GAA CAA TTT TCC TCA AAG TTG TAG
D-R CG GGATCC AGA AAA TGT AAA GAT TGA TAA TGG
CaMV35S-F GAA TGC TAA CCC ACA GAT GGTTAG
GUS-R GTT CAA CGC TGA CAT CAC CAT TG
CYC-B-F GGG TAA TGA GCC ATA TTT AAG GG
CYC-B-R TAG GAT CCA GAT CAA AGA AAG CG
EFlα-F TGG TCATTG GTC ATG TTG A
EFlα-R GCA GAT CAT TTG CTT GAC ACCAAG