Table 2 Primers used in this study (Incorporated restriction site sequences are underlined)
Primer | Sequence (5'-3') |
|---|---|
Walker1 | CTA ATA CGA CTC ACT ATA GGG NNN NA TGC |
Walker2 | CTA ATA CGA CTC ACT ATA GGG NNN NT AGC |
Walker3 | CTA ATA CGA CTC ACT ATA GGG NNN NG ATC |
Walker4 | CTA ATA CGA CTC ACT ATA GGG NNN NC TAG |
Adaptor walker | CTA ATA CGA CTC ACT ATA GGG |
R1 | GAT AAT GAT CAC GTC GAA TTG AG |
R2 | CTC TGG CTT TGA TGT GGGT GCT |
D0-F | CCC AAGCTT GAC TTC ACC CTT CTT TCT TGT C |
D1-F | CCC AAGCTT GAT CTA CTG GAA ATT GAC CAA C |
D2-F | CCC AAGCTT TCA CTA TGT TTG TTA GTG CTT G |
D3-F | CCC AAGCTT GAA CAA TTT TCC TCA AAG TTG TAG |
D-R | CG GGATCC AGA AAA TGT AAA GAT TGA TAA TGG |
CaMV35S-F | GAA TGC TAA CCC ACA GAT GGTTAG |
GUS-R | GTT CAA CGC TGA CAT CAC CAT TG |
CYC-B-F | GGG TAA TGA GCC ATA TTT AAG GG |
CYC-B-R | TAG GAT CCA GAT CAA AGA AAG CG |
EFlα-F | TGG TCATTG GTC ATG TTG A |
EFlα-R | GCA GAT CAT TTG CTT GAC ACCAAG |