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Table 2 Primers used in this study (Incorporated restriction site sequences are underlined)
| Primer | Sequence (5'-3') |
|---|---|
| Walker1 | CTA ATA CGA CTC ACT ATA GGG NNN NA TGC |
| Walker2 | CTA ATA CGA CTC ACT ATA GGG NNN NT AGC |
| Walker3 | CTA ATA CGA CTC ACT ATA GGG NNN NG ATC |
| Walker4 | CTA ATA CGA CTC ACT ATA GGG NNN NC TAG |
| Adaptor walker | CTA ATA CGA CTC ACT ATA GGG |
| R1 | GAT AAT GAT CAC GTC GAA TTG AG |
| R2 | CTC TGG CTT TGA TGT GGGT GCT |
| D0-F | CCC AAGCTT GAC TTC ACC CTT CTT TCT TGT C |
| D1-F | CCC AAGCTT GAT CTA CTG GAA ATT GAC CAA C |
| D2-F | CCC AAGCTT TCA CTA TGT TTG TTA GTG CTT G |
| D3-F | CCC AAGCTT GAA CAA TTT TCC TCA AAG TTG TAG |
| D-R | CG GGATCC AGA AAA TGT AAA GAT TGA TAA TGG |
| CaMV35S-F | GAA TGC TAA CCC ACA GAT GGTTAG |
| GUS-R | GTT CAA CGC TGA CAT CAC CAT TG |
| CYC-B-F | GGG TAA TGA GCC ATA TTT AAG GG |
| CYC-B-R | TAG GAT CCA GAT CAA AGA AAG CG |
| EFlα-F | TGG TCATTG GTC ATG TTG A |
| EFlα-R | GCA GAT CAT TTG CTT GAC ACCAAG |
