Figure 4From: Transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in Arabidopsis thalianareveal the involvement of hormone and defense signaling pathways Ethylene reporter gene expression in plants treated with phenanthrene and ACC, and grown in the dark. Histochemical staining of GUS activity in CH5B::GUS (A-D) and AtGSTF2::GUS (E-H) transgenic Arabidopsis plants grown for 4 d in the dark. A and E, 0 mM phenanthrene, 0 μM ACC; B and F, 0 mM phenanthrene, 20 μM ACC; C and G, 0.25 mM phenanthrene and 0 μM ACC; D and H, 0.25 mM phenanthrene, 20 μM ACC. Seedlings were stained for 15 h for GUS activity in staining buffer containing 2 mM 5-bromo-4-chloro-3-indolyl-b-D-glucuronide. Scale bars 1 mm.Back to article page