Phenotypes of the AtYLMG1-1 overexpressers. (A) Three-week-old seedlings of the FOX line (FN026 and FN028), and plants with a 35S promoter-At3g07430 transgene (35S-AtYLMG1-1). Chloroplasts in single leaf mesophyll cells of FN026, FN028, and the 35S-AtYLMG1-1 transgenic plant. Bars = 10 mm (left) and 10 μm (right). (B) The average of the chloroplast diameter is shown in each graph along with the standard deviation. n = 50. (C) Levels of the AtYLMG1-1 transcript in the wild type, FOX lines and the 35S-AtYLMG1-1 transgenic plants. Transcript levels were analyzed by RT-PCR in the wild type (lane 1 and 5), FN026 (lane 2 and 6), FN028 (lane 3 and 7), and the 35S-AtYLMG1-1 transgenic plants (lane 4 and 8). A micro litter (lane 1-4) or 0.1 μl (lane 5-8) of reverse-transcription product was used as the PCR template. GAPDH was used as the quantitative control. Triangle indicates the RT-PCR products of AtYLMG1-1, and asterisk indicates that of GAPDH. (D) Levels of the AtYLMG1-1 protein in the wild type, FOX line, and the 35S-AtYLMG1-1 transgenic plants. Total proteins extracted from 3-week-old seedling of the wild type (WT), FOX line (FN028), and the 35S-AtYLMG1-1 transgenic plants (35S-AtYLMG1-1) were analyzed with the anti-AtYLMG1-1 antibodies raised against a peptide fragment of AtYLMG1-1. Fifty micrograms of proteins were loaded in each lane. The Rubisco small subunit (Rubisco SSU) was detected by Coomassie brilliant blue (CBB) staining as the quantitative control. (E) Localization of FtsZ in the wild type and the AtYLMG1-1 overexpresser. Localization of FtsZ2-1 in mesophyll cells was examined under immunofluorescence microscopy. The green fluorescence shows the localization of FtsZ2-1 and the autofluorescence of chlorophyll is depicted in red. Bars = 5 μm.