Table 3 Characteristics of pigeonpea EST-SSR markers
Primer ID | SSR motif | Tm (°C) | Product size (bp) | No. of alleles | PIC value |
|---|---|---|---|---|---|
ICPeM0001 | (A)56ttg(A)30 | 60 | 240 | 1 | 0.00 |
ICPeM0003 | (A)23n(A)11n(C)12 | 60 | 150 | 7 | 0.79 |
ICPeM0005 | (A)99n(C)11 | 60 | 280 | 5 | 0.35 |
ICPeM0006 | (T)37gg(T)56 | 60 | 246 | 1 | 0.00 |
ICPeM0009 | (A)85g(A)27 | 60 | 208 | 1 | 0.00 |
ICPeM0010 | (T)11n(T)20 | 60 | 266 | 1 | 0.00 |
ICPeM0011 | (A)58gggg(A)24 | 60 | 280 | 1 | 0.00 |
ICPeM0013 | (A)87g(A)29n(C)11 | 62 | 150 | 4 | 0.66 |
ICPeM0017 | (AG)8n(AT)8 | 59 | 240 | 1 | 0.00 |
ICPeM0018 | (A)10taca(T)12 | 59 | 90 | 1 | 0.00 |
ICPeM0019 | (TTA)7n(T)12 | 60 | 236 | 1 | 0.00 |
ICPeM0023 | (A)128n(C)11n(C)11 | 60 | 279 | 1 | 0.00 |
ICPeM0024 | (A)11cccg(A)10 | 60 | 279 | 1 | 0.00 |
ICPeM0025 | (A)10n(C)11n(C)11n(C)12 | 61 | 223 | 1 | 0.00 |
ICPeM0028 | (A)58cc(A)27 | 61 | 239 | 1 | 0.00 |
ICPeM0029 | (A)57t(A)30 | 60 | 184 | 1 | 0.00 |
ICPeM0030 | (A)52tt(A)28 | 61 | 252 | 1 | 0.00 |
ICPeM0031 | (A)13gn(A)67n(A)19 | 60 | 279 | 1 | 0.00 |
ICPeM0033 | (A)12tt(A)12n(A)13 | 60 | 350 | 7 | 0.31 |
ICPeM0034 | (A)13n(AT)9 | 60 | 236 | 1 | 0.00 |
ICPeM0035 | (T)21n(A)11 | 60 | 218 | 1 | 0.00 |
ICPeM0038 | (C)15acctcactaaccaaact(C)10 | 60 | 266 | 1 | 0.00 |
ICPeM0039 | (G)10n(T)94 | 59 | 262 | 1 | 0.00 |
ICPeM0041 | (T)12n(A)10 | 60 | 310 | 4 | 0.48 |
ICPeM0047 | (T)18c(T)27 | 60 | 213 | 1 | 0.00 |
ICPeM0050 | (A)112n(C)13n(C)11 | 63 | 264 | 1 | 0.00 |
ICPeM0052 | (C)12tccctcctctcgccca(C)12 | 60 | 233 | 1 | 0.00 |
ICPeM0053 | (C)24t(C)26 | 60 | 136 | 1 | 0.00 |
ICPeM0054 | (G)10agccc(G)10 | 60 | <90 | 1 | 0.00 |
ICPeM0060 | (T)13c(T)10 | 60 | 150 | 1 | 0.00 |
ICPeM0061 | (T)19n(A)28 | 59 | 243 | 1 | 0.00 |
ICPeM0064 | (ATT)7(T)10 | 59 | 300 | 3 | 0.49 |
ICPeM0065 | (A)10(AT)9 | 60 | 90 | 1 | 0.00 |
ICPeM0066 | (AT)9 | 60 | 310 | 3 | 0.34 |
ICPeM0067 | (TA)11 | 60 | 200 | 3 | 0.29 |
ICPeM0068 | (GT)11 | 60 | 260 | 4 | 0.26 |
ICPeM0069 | (AT)8 | 60 | <90 | 1 | 0.00 |
ICPeM0070 | (AT)8 | 61 | 310 | 1 | 0.00 |
ICPeM0071 | (GA)9 | 61 | 190 | 5 | 0.64 |
ICPeM0072 | (AT)8 | 60 | <90 | 1 | 0.00 |
ICPeM0073 | (AG)9 | 60 | <90 | 1 | 0.00 |
ICPeM0074 | (AGA)6 | 60 | 300 | 1 | 0.00 |
ICPeM0075 | (ACA)6 | 60 | 300 | 2 | 0.38 |
ICPeM0076 | (CTT)6 | 60 | 200 | 1 | 0.00 |
ICPeM0077 | (AAT)7 | 60 | 310 | 1 | 0.00 |
ICPeM0078 | (GCC)6 | 60 | 320 | 3 | 0.24 |
ICPeM0079 | (ATT)6 | 60 | 250 | 4 | 0.40 |
ICPeM0080 | (TGGAC)5 | 60 | 200 | 1 | 0.00 |
ICPeM0081 | (TAAT)5 | 60 | 300 | 1 | 0.00 |
ICPeM0082 | (AT)9 | 60 | 200 | 3 | 0.27 |
ICPeM0083 | (AG)9 | 60 | 190 | 1 | 0.00 |
ICPeM0084 | (TATG)6 | 60 | 240 | 3 | 0.59 |