Figure 11
A snapshot of sequence alignment of EST sequences and amplicons for contig 433 validating the in silico predicted SNP. CAP3 alignment of ESTs (a) generated from 'ICP 7035' and 'TTB 7'in contig 433 showing a SNP between these genotypes and (b) Multiple sequence alignment of amplicon sequences generated from genomic DNA of four genotypes ('ICPL 20102', 'ICP 2376', 'ICP 7035' and 'TTB 7') with the primer pairs for the assembled contig 433. The in silico identified SNP in the EST contig 433 was confirmed in the amplicon sequences.