The 77 K fluorescence emission ratio F733/F685 and the thylakoid protein phosphorylation in WT and the DnaJ mutants. A, F733/F685 ratio in WT and the DnaJ mutants after 6 h treatment of plants under different light conditions. The values are means ± SD (n = 9~12) of three independent experiments with 3 to 4 replicates. B, Phosphorylation levels of thylakoid proteins after similar light treatments of plants as in A. C, Changes in thylakoid protein phosphorylation during a long-term high light (1000 μmol photons m-2 s-1) treatment. Thylakoid membranes were isolated from leaves after treatment of plants in darkness and after illumination at growth light and high light conditions for time periods indicated. 1.0 μg of chlorophyll was loaded to the wells for immunoblotting with p-thr antibody. WT, wild-type; D, darkness; GL, 120 μmol photons m-2 s-1 growth light; HL-500, 500 μmol photons m-2 s-1high light; HL-1000, 1000 μmol photons m-2 s-1 high light.