Capacity of CO
assimilation in DnaJ mutants and WT. A, Light response curves; B, CO2 response curves; C, A-Ci curves which based on intracellular CO2 concentration less than 300 μmol mol-1; D, Immunoblot analysis of Rubisco Activase, Rubisco large subunit (Rubisco LU) and small subunit (Rubisco SU) in leaves collected from growth light conditions and from darkness. Total proteins were isolated from leaves after 6 h illumination under growth light and in the end of the diurnal dark period. 10 μg of leaf total proteins was loaded. Protein quantification (indicated below the blots as a percentage of protein from that present in WT in the light) is based on three independent immunoblot experiments (mean ± SD). A, CO2 assimilation; Ci, intracellular CO2 consentration; PPFD, photosynthetic photon flux density. WT, wild-type; GL, growth light (120 μmol photons m-2 s-1).