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Table 4 Differentially accumulated transcripts between LoPro and HiPro identified by Illumina® high-throughput transcriptome sequencing

From: Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean

#

Comparison

Sequence ID

A

B

Ch

Start

End

Strand

BlastP Description

E-value

1

Overall

Glyma20 g18880

51

0

Gm20

26510968

26513359

-

Mov34-1

2 × 10-21

 

Stage 3

Glyma20 g18880

15

0

Gm20

26510968

26513359

-

"

"

 

Stage 4

Glyma20 g18880

18

0

Gm20

26510968

26513359

-

"

"

2

Stage 4

Glyma20 g19620

268

106

Gm20

27706435

27707431

+

no alignments with E-value < 10-10

na

3

Stage 4

Glyma20 g19630

259

95

Gm20

27706477

27706935

-

no alignments with E-value < 10-10

na

4

Overall

Glyma20 g19680

24

2

Gm20

27899125

27899596

-

Hsp22.5

5 × 10-72

 

Stage 3

Glyma20 g19680

24

0

Gm20

27899125

27899596

-

"

"

5

Overall

Glyma20 g21030

61

12

Gm20

29984895

29986397

+

Putative ammonium transporter AMT1

0

 

Stage 1

Glyma20 g21030

36

4

Gm20

29984895

29986397

+

"

"

6

Overall

Glyma20 g21040

40

8

Gm20

29984951

29986210

-

no alignments with E-value < 10-10

na

 

Stage 1

Glyma20 g21040

27

4

Gm20

29984951

29986210

-

"

"

7

Overall

Glyma20 g21080

13

0

Gm20

30044891

30045091

+

ATP synthase D chain

4 × 10-17

8

Overall

Glyma20 g21140

76

0

Gm20

31078277

30182887

-

no alignments with E-value < 10-10

na

 

Stage 1

Glyma20 g21140

13

0

Gm20

30178277

30182887

-

"

"

 

Stage 3

Glyma20 g21140

38

0

Gm20

30178277

30182887

-

"

"

 

Stage 4

Glyma20 g21140

15

0

Gm20

30178277

30182887

-

"

"

9

Overall

Glyma20 g21540

32

0

Gm20

30873568

30873806

+

Putative uncharacterized protein

2 × 10-21

 

Stage 3

Glyma20 g21540

13

0

Gm20

30873568

30873806

+

"

"

10

Stage 3

Glyma20 g21780

121

36

Gm20

31386550

31389333

+

Ethylene receptor

0

11

Stage 3

Glyma20 g22170

142

46

Gm20

32098751

32103750

+

Glutamyl-tRNA synthetase

0

12

Stage 1

Glyma20 g22650

9

42

Gm20

32589230

32589715

+

no alignments with E-value < 10-10

na

  1. The sequence identifier (ID) is shown for each numbered gene candidate with differentially accumulated transcripts between genotypes at the LG I protein QTL region. Transcript sequencing read counts for LoPro and HiPro are reported along with the sequence location for the closest predicted gene. The BlastP description is reported for each gene at E-value < 10-10. na = not applicable.