Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida

Table 3 Gene suitability rankings for the whole dataset.

Rank position	NormFinder		BestKeeper		qBasePlus M		qBasePlus CV		geNorm M		Consensus
	Mitchell	V30	Mitchell	V30	Mitchell	V30	Mitchell	V30	Mitchell	V30	*Mitchell*	*V30*
1	EF1α	UBQ	CYP	CYP	EF1α	ACT	EF1α	RAN1	RAN1	RPS13	EF1alpha	CYP
2	CYP	RAN1	EF1α	EF1α	SAND	RAN1	SAND	CYP	SAND	UBQ	SAND	RAN1
3	RPS13	ACT	RPS13	ACT	RAN1	CYP	RPS13	ACT	UBQ	RAN1	RPS13	ACT
4	UBQ	GAPDH	ACT	SAND	RPS13	TUB	RAN1	TUB	EF1alpha	CYP	RAN1	UBQ
5	ACT	RPS13	UBQ	UBQ	CYP	RPS13	CYP	RPS13	RPS13	ACT	CYP	RPS13
6	SAND	SAND	TUB	GAPDH	UBQ	UBQ	UBQ	UBQ	CYP	TUB	UBQ	TUB
7	TUB	EF1alpha	SAND	RPS13	TUB	EF1alpha	TUB	EF1alpha	TUB	EF1alpha	TUB	EF1alpha
8	GAPDH	TUB	RAN1	RAN1	ACT	SAND	ACT	GAPDH	ACT	SAND	ACT	SAND
9	RAN1	CYP	GAPDH	TUB	GAPDH	GAPDH	GAPDH	SAND	GAPDH	GAPDH	GAPDH	GAPDH

Gene expression data were analyzed using five statistical parameters in both Petunia lines. Each column refers to a gene suitability ranking computed by one statistical tool, taking into account all data of a Petunia line.

ISSN: 1471-2229