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Table 2 Optimal genes for quantification of individual and mixed organs in each Petunia line.

From: Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida

Statistic Flower Leaf
Mitchell
  A B C D A+B+C+D A C A+C
M geNorm EF1α (0.05) RPS13 (0.05) SAND (0.14) UBQ (0.14) EF1α (0.13) RPS13 (0.13) RAN1 (0.08) RPS13 (0.08) RAN1 (0.47) SAND (0.47) EF1α (0.11) RPS13 (0.11) RAN1 (0.14) SAND (0.14) EF1α (0.37) RPS13 (0.37)
M qBasePlus ACT (0.55) RPS13 (0.56) EF1α (0.60) RPS13 (0.60) CYP (0.60) SAND (0.64) EF1α (0.30) RAN1 (0.34) EF1α (0.80) SAND (0.82) RPS13 (0.77) EF1α (0.78) SAND (0.93) RAN1 (0.93) EF1α (0.85) RAN1 (0.92)
CV qBasePlus ACT (0.05) SAND (0.15) RPS13 (0.07) CYP (0.25) CYP (0.09) SAND (0.11) EF1α (0.05) TUB (0.14) EF1α (0.25) SAND (0.28) RPS13 (0.14) RAN1 (0.18) SAND (0.12) RAN1 (0.18) RAN1 (0.20) EF1α (0.21)
Min. number 2 (0.04) 2 (0.07) 2 (0.12) 2 (0.05) 4 (0.15) 2 (0.10) 2 (0.13) 2 (0.12)
V30
Statistic Flower Leaf
  A B C D A+B+C+D A C A+C
M geNorm RAN1 (0.11) UBQ (0.11) TUB (0.12) CYP (0.12) RPS13 (0.23) UBQ (0.23) ACT (0.02) CYP (0.02) RAN1 (0.45) ACT (0.45) TUB (0.07) RAN1 (0.07) RPS13 (0.09) TUB (0.09) RAN1 (0.20) UBQ (0.20)
M qBasePlus CYP (0.30) RAN1 (0.33) CYP (0.66) TUB (0.69) SAND (0.63) CYP (0.63) RPS13 (0.29) EF1α (0.30) ACT (2.27) RAN1 (2.44) TUB (0.34) UBQ (0.36) SAND (0.27) RPS13 (0.29) UBQ (0.49) RPS13 (0.51)
CV qBasePlus CYP (0.05) TUB (0.10) RAN1 (0.09) CYP (0.11) SAND (0.10) ACT (0.25) EF1α (0.06) CYP (0.06) ACT (0.70) RAN1 (0.82) UBQ (0.06) TUB (0.09) SAND (0.03) RPS13 (0.06) UBQ (0.14) RPS13 (0.16)
Min. number 2 (0.10) 2 (0.07) 2 (0.09) 2 (0.04) NA 2 (0.04) 2 (0.03) 2 (0.07)
  1. M values computed by geNorm and qBasePlus allow to rank optimal reference genes. For each organ and mix of organs the two top-ranked genes are shown. The number of genes required for a reliable quantification is established using a Pairwise Variation (PV) cut-off of 0.15; n is the the minimum number of control genes required NA means that no one pairwise variation was under the proposed cut-off.