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Table 2 Probes used in the RFLP analysis to analyse different mitochondrially-encoded genes

From: An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica

Gene probe Source Fragment size Description
atp9 Restorer rice line 263 bp Complete coding region cloned as a BamHI-SacI fragment
atpA Restorer rice line 720 bp Partial coding region (lacking C-terminus) cloned as a PstI-BamHI fragment
atp6 Restorer rice line 876 bp
350 bp
Partial coding region (lacking C-terminus) cloned as a BamHI-BglII fragment.
Partial 3' UTR cloned as EcoRI-HindIII fragment.
orfB Restorer rice line 468 bp Complete coding region cloned as a BamHI-SacI fragment.
  1. These gene probes were amplified from the restorer rice line by PCR, then cloned and sequenced.