Table 2 Probes used in the RFLP analysis to analyse different mitochondrially-encoded genes
Gene probe | Source | Fragment size | Description |
|---|---|---|---|
atp9 | Restorer rice line | 263 bp | Complete coding region cloned as a BamHI-SacI fragment |
atpA | Restorer rice line | 720 bp | Partial coding region (lacking C-terminus) cloned as a PstI-BamHI fragment |
atp6 | Restorer rice line | 876 bp 350 bp | Partial coding region (lacking C-terminus) cloned as a BamHI-BglII fragment. Partial 3' UTR cloned as EcoRI-HindIII fragment. |
orfB | Restorer rice line | 468 bp | Complete coding region cloned as a BamHI-SacI fragment. |