Figure 2

RFLP analysis of sterile, maintainer and restorer rice lines for orfB and atp6 genes. 2A. Southern blot analysis of the APMS-6A WA sterile line (lanes: 1, 3, 5, 7, 9) along with corresponding maintainer APMS-6B (lanes: 11, 12, 13, 14, 15) and restorer BR-1870 (lanes: 2, 4, 6, 8, 10) lines. The same blots that were shown in Figure 1 were stripped and re-probed with the CDS of the orfB gene. 2B. DNA Gel Blot Analysis of WA-CMS line IR58025A(s), IR58025B(m) and its restorer(r). a. Mitochondrial DNA digested with EcoRI restriction enzyme and probed with rice orfB CDS. b. Same blot stripped and probed with atp6 partial CDS. 2C. DNA Gel Blot Analysis of non WA-CMS rice line, Kalinga-32A and corresponding fertile maintainer line, Kalinga-32B Kalinga-32A (lane 1, 3, & 5) and Kalinga-32B (lane 2, 4, & 6) mitochondrial DNA (10 μg) digested with three different restriction enzymes, viz., EcoRI (lanes 1 & 2), BglII (lanes 3 & 4) and ScaI (lane 5 & 6), were electrophoresed, blotted and probed with rice atp6 CDS. Same blot probed with orfB CDS.