Figure 14

OrfB gene 1.1 kb transcript specific RT-PCR from 6AR F 2 sterile and fertile plants. Ethidium bromide stained agarose gel (1%) showing the RT-PCR products using gene specific primers Mtg-1 and Corf from the F2 plants. The electropherograms show: (A) all the five F2 sterile plants identified; (B) 13 fertile plants as representative of 24 F2 fertile plants that were analyzed. DNA molecular weight marker used (pUC18/HinfI).