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Figure 1 | BMC Plant Biology

Figure 1

From: An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica

Figure 1

RFLP analysis of sterile, maintainer and restorer rice lines for atp9 , atpa and atp6 genes. Southern blot analysis of the APMS-6A WA sterile line (lanes: 1, 3, 5, 7, 9) along with the corresponding maintainer APMS-6B (lanes: 11, 12, 13, 14, 15) and restorer BR-1870 (lanes: 2, 4, 6, 8, 10) lines. Mitochondrial genomic DNA (10 μg per lane) was digested with different restriction enzymes, viz., BglII (lanes: 1, 2, 12), ScaI (lanes 3, 4, 15), DraI (lanes 5, 6, 13), EcoRI (lanes 7, 8, 11) &HindIII (lanes 9, 10, 14), run on an 0.8% agarose gel, blotted and probed with different mitochondrially-encoded CDSs or partial CDSs. Lane M: EcoRI and HindIII-digested phage λ DNA (molecular weight marker). Panel A: Southern blots probed with the entire CDS of the atp9 gene. Panel B: The same blots were stripped and re-probed with the partial CDS of the atpA gene. Panel C: The same blots were re-probed with the partial CDS of the atp6 gene. Panel D: The same blots were re-probed with the 3'UTR of the atp6 gene.

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BMC Plant Biology

ISSN: 1471-2229

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