Sequence comparison of both alleles of the Rfo locus. L7rfo is the sequence determined in this study (accession number FN397617) and D81Rfo is the corresponding region from the BAC64 clone (accession number AJ550021) . A- Dot Matrix view of the YASS comparison of the two sequences using default parameters except that the E-value threshold was 10-10. B- Schematic representation of both alleles. Black stars indicate the positions targeted by PCR markers used to screen the BAC library. The dark grey boxes highlight collinear and highly similar regions. The hatched boxes indicate regions that are homologous but not collinear. The white boxes indicate the L7rfo region that has no corresponding sequence on D81Rfo, and vice-versa. Arrows symbolize genes predicted by GENESCAN and indicate the direction of transcription. Unlabelled predicted genes on D81Rfo are highly similar to the corresponding genes on L7rfo, and were previously reported by . Gene information is provided in Table 1.