STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro

Table 2 Biochemical characterization of STIL.

Treatment	LePRK2 dephosphorylation activity
1.5 N HCl (microwave)	-
1 N HCl 100°C 4 or 20 h	+
1 N NaOH 100°C 2 h	+
50 mM DTT 100°C 15 min	+
Pepsin	+
Carboxypeptidase Y	+
Trypsin	+
Proteinase K	+
Lyticase	+
Pectolyase	+

STIL was subjected to the listed treatments and the dephosphorylating activity was tested in a LePRK2 dephosphorylation assay (as described in Methods). STIL resistance to the treatment is indicated by "+", while "-" indicates STIL loss of activity.

ISSN: 1471-2229