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Figure 2 | BMC Plant Biology

Figure 2

From: Novel induced mlo mutant alleles in combination with site-directed mutagenesis reveal functionally important domains in the heptahelical barley Mlo protein

Figure 2

Yemenite accessions 5589 and 5590 harbor the natural mlo -11 allele. Genomic DNA of the indicated barley lines was used as a template for PCR amplification (40 cycles; extension 1 minute at 72°C) using either the oligonucleotide combination ADUP7A/Mlo6 (diagnostic for the presence of the mlo-11 repeat structure [11]) or Mlo6/Mlo10 (indicating presence/absence of a MITE associated with the 5'-terminal repeat in the majority of mlo-11 haplotypes [11, 30]). PCR products were separated by agarose gel electrophoresis and visualized via ethidium bromide staining in combination with UV transluminescence. Cultivar Ingrid (Mlo genotype) and line back cross Ingrid (BCI) mlo-11 served as controls for wild type and mlo-11 genotypes, respectively. Note that lines RAH995 and Ab1089 were included as additional controls.

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BMC Plant Biology

ISSN: 1471-2229

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