Figure 4

CPNs deliver siGLO Red small RNAs to protoplasts. Bright field and epifluorescence images of protoplasts treated with: (A, B, C) 25 μM CPNs and 200 nM siGLO Red small RNAs (red); (D, E) only 200 nM siGLO Red small RNAs; (F, G) untreated protoplasts (negative controls). (E) Image shows no uptake of small RNAs in the absence of CPNs. (G) Image shows no green fluorescence, as expected. Experiments were repeated 2-3 times. Scale bars equal 20 μm.