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Figure 3 | BMC Plant Biology

Figure 3

From: Rice Hypersensitive Induced Reaction Protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death

Figure 3

Hypersensitive response lesions and spontaneous cell death due to the overexpression of OsHIR1. (a) Hypersensitive response lesions in some OsHIR1 transgenic plants. Three weeks after germination, white necrotic lesions located randomly at the margins and tips of leaves (red arrows) were observed in about 20% of the OsHIR1 transgenic plants. Such a phenomenon was not found in untransformed wild type (Col-0), empty vector transgenic control (Col-0/V7), or OsLRR1 transgenic plants (Col-0/OsLRR1). (b) Lactophenol-trypan blue staining showing spontaneous cell death. Leaves of 3-week-old plants were stained with lactophenol-trypan blue to detect dead cells. Spontaneous cell death found on the leaves of OsHIR1 and OsLRR1 transgenic plants were indicated by black arrows. Bars = 100 μm

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