Table 1 Quantitative reverse transcriptase-PCR primers used for detecting expressions from wheat polyamine biosynthesis genes.
Gene | Forward primer (5'-3') | Reverse primer (5'-3') | Locus | GenBank accession |
|---|---|---|---|---|
Ornithine decarboxylase (ODC) | AGCGTTACTTCGGGGAGCTT | ATTGTGAAGGCGGTCTCG | Os09g37120 | HM770451 |
Arginine decarboxylase 1 (ADC1) | CACCAAGATACCAGGCCACT | GTGGAAGTGCAGCAACTTGA | Os04g01690 | HM770446 |
Arginine decarboxylase 2 (ADC2) | AGGAGGAGGAGCTCGACATT | GCCGAACTTGCCCTTCTC | Os06g04070 | HM770449 |
Agmatinase/Arginase | GGGAAGAGATTTGGTGTGGA | TCACACCTTCCCCAAGTTTC | Os04g01590 | HM770450 |
S-adenosylmethionine decarboxylase 2 | GCGTCCTCATCTACCAGAGC | CTTGCCTTCCTTGACCAGAG | Os04g42090 | HM770448 |
Spermidine synthase 1 | TGATTCAGGACATGCTTTCG | CCCAATTGCACCACTAGGAT | Os02g15550 | HM770442 |
Spermidine synthase 2 | GCAAAAATCCAATGACACCA | TGTGGCTGCACACACATCTA | Os06g33710 | HM770452 |
Argininosuccinate synthase | ACGCTGAAGGTTTCATCAGG | TAGATGCCCTTCTCCAGCAT | Os12g13320 | HM770445 |
Argininosuccinate lyase | GTTGAACAGTTGGAGCGTGA | GAGTCCAGTTCCAGCCAAAG | Os03g19280 | HM770447 |
Carbamoyl-phosphate synthase | TTGGAAAATTGTTGGTGTTG | CCCATTCATATGGAGCATC | Os02g47850 | HM770444 |
Ornithine carbamoyl transferase | ATGAAGCCCTGATGGAGATG | ATGGCACCGTCTGTTACCTC | Os02g47590 | HM770443 |
18 S rRNA* | CGACCTACTCGACCCTTCGGCCGG | CGATGCCGGAAACACGACCCGG | - | Â |
Peroxidase†(TaPERO) | GAGATTCCACAGATGCAAACGAG | GGAGGCCCTTGTTTCTGAATG | - | X56011 |
18 S rRNA fungal* | GTCCGGCCGGGCCTTTCC | AAGTCCTGTTTCCCCGCCACGC | - | Â |
TRI5* | CACTTTGCTCAGCCTCGCC | CGATTGTTTGGAGGGAAGCC | FGSG_03537 | Â |