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Figure 3 | BMC Plant Biology

Figure 3

From: HvCEBiP, a gene homologous to rice chitin receptor CEBiP, contributes to basal resistance of barley to Magnaporthe oryzae

Figure 3

Sequence and expression profiling of HvCEBiP. (A) Alignment of the amino acid sequences between rice CEBiP (Rice) and barley HvCEBiP (Barley). Putative coding sequence of HvCEBiP was aligned with rice CEBiP. Identical amino acids are highlighted with black boxes. SP, signal peptide; LysM 1/LysM 2, LysM motif; TM, transmembrane region. Arrows indicated primer position used for gene silencing of HvCEBiP. (B) Expression profiling of HvCEBiP and several defense-related genes. Conidial suspensions (1 × 105 conidia/ml) of the wild-type strain Hoku-1 or mossd1 mutant K1 were spotted onto barley leaves and total RNAs were extracted from inoculated tissues at 0 (no inoculation), 3, 6, 12, 24 and 48 hpi for RT-PCR. The expression of HvCEBiP was detectable with similar transcript levels at all time points in the leaves inoculated with either Hoku-1 or K1. The expression of HvPAL, HvRBOHA and HvPR-5 was detectable at all time points, but expression of HvPR-1 and HvPR-2a was induced after inoculation with M. oryzae. For checking genomic contamination, PCR of HvEF1α was performed using total RNA as template (designated as RT-). Ribosomal RNAs are presented as loading control.

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