Figure 8

Chilling sensitivity of svr3. (A) Phenotypes of seven-week-old wild-type, var2-5, TAG-11 and svr3-1. Plants were germinated and maintained at 22°C for three weeks before subjected to the chilling treatment at 8°C for four weeks. (B) Chlorophyll accumulation in the emerging yellow leaf tissues of the mutant and emerging green leaf tissues of wild-type (*: p < 0.01). (C)-(E) Accumulation patterns of chloroplast rRNA transcripts at 8°C. Northern blots of 23 S (C), 4.5 S (D), and 16 S (E) rRNAs were carried out with total RNA samples extracted from the same tissues as in (B). Northern blot analysis with indicated probes was performed as in Figure 6. Gel loading controls are shown in Additional file 1, Figure S5.