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Figure 7 | BMC Plant Biology

Figure 7

From: A var2 leaf variegation suppressor locus, SUPPRESSOR OF VARIEGATION3, encodes a putative chloroplast translation elongation factor that is important for chloroplast development in the cold

Figure 7

Accumulation of chloroplast proteins at 22°C. Total leaf proteins were extracted from two-week-old seedlings of wild-type, var2-5, TAG-11 (var2-5 svr3-1), svr3-1 and svr3-2 grown under the same conditions as in Figure 1A. A dilution series of the wild-type samples were loaded. Other samples were standardized to equal amounts of fresh tissue. Immunoblots were performed using polyclonal antibodies against chloroplast proteins of representative complexes: FtsH complex (VAR2, AtFtsH1), PSII (D1, PsbP), PSI (PsaF, PsaN), ATP synthase (ATPα), Rubisco (large subunit [LS]), Light harvesting complex (Lhcb2) and Cytochrome b6f (Rieske Fe-S). Plastid encoded proteins are D1, ATPα and Rubisco large subunit (LS). Nuclear encoded proteins are VAR2, AtFtH1, PsbP, PsaF, PsaN, Lhcb2 and Rieske Fe-S.

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