Figure 4

Bioinformatics analysis of SVR3. (A) Domain architecture of translation elongation factor GTPases. Chloroplast transit peptides (CTP) were predicted by TargetP [42]. Conserved domains were identified using InterProScan http://www.ebi.ac.uk/Tools/InterProScan/[82]. Arabidopsis protein sequences were obtained from TAIR http://www.Arabidopsis.org. E. coli protein sequences were obtained from uniprot.org (Accession numbers: EF-Tu, P0A6N1; EF-G, P0A6M8; LepA, P60785; TypA, P32132). (B) Phylogenetic tree of TypA homologs from Arabidopsis, rice, Chlamydomonas reinhardtii, Synechocystis sp. PCC6803 and E. coli. Full length protein sequences were obtained from the National Center for Biotechnology Information (NCBI). Gene ID or Genbank accession number is listed in the figure. MEGA4 software [83] was used for sequence analysis and phylogenetic tree construction. (C) Conservation of TypA-like gene structures in Arabidopsis and rice. Gene models were constructed based on annotation of the Arabidopsis and rice genomes. Boxes represent exons and lines represent introns. 5' and 3' untranslated regions (UTRs) are shaded. Numbers above each box refer to the number of nucleotides of each exon excluding the UTRs.