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Figure 2 | BMC Plant Biology

Figure 2

From: A var2 leaf variegation suppressor locus, SUPPRESSOR OF VARIEGATION3, encodes a putative chloroplast translation elongation factor that is important for chloroplast development in the cold

Figure 2

Cloning of SVR3. (A) Procedure of map-based cloning of SVR3 is described in Methods. Markers used in fine mapping are listed in Additional file 1, Table S1. A total of 570 F2 plants (1140 chromosomes) were examined, and the number of recombinants is shown under each marker. The position of SVR3 (At5g13650) is indicated by the asterisk. In the gene model, boxes represent exons while solid lines represent introns. Shaded parts represent the 5' and 3' untranslated regions (UTRs). (B) and (C) Verification of the identity of SVR3 using PCR (B) and RT-PCR (C). Primers used for PCR and RT-PCR are indicated by arrows in gene model in (A).

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