Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamianaby cDNA-amplified fragment length polymorphism

Table 3 BaMV coat protein accumulation in TRV-driven gene-silenced Nicotiana benthamiana plants.

TDF^a	Expression^b	Protein candidate	CP^c	Significance^d
ACCT13	+	regulator of gene silencing [N. tabacum]	72 ± 3
ACGT4	+	thioredoxin h-like protein [N. tabacum]	181 ± 43	**
ACGT11-1	+	plasma membrane polypeptide [N. tabacum]	172 ± 49	**
ACAG2-1	+	NEP1-interacting protein 2 [Arabidopsis thaliana]	191 ± 14	***
ACCA10	+	AER [N. tabacum]	77 ± 32
ACCT1-1	-	CDK-activating kinase[Candida dubliniensis CD36]	197 ± 25	**
ACCT5-1	-	lysine ketoglutarate reductaselysine trans-splicing [Zea mays]	74 ± 24
ACAG8	-	chloroplast carbonic anhydrase [N. benthamiana]	68 ± 14	*
ACGT2-1	-	photosystem II 23 kDa polypeptide [N. tabacum]	149 ± 40	**
ACAG1	-	ferredoxin--NADP(+) reductase [N. tabacum]	190 ± 24	**
ACGT12	-	lipid transferase [N. tabacum]	42 ± 18	***
ACTC5-1	-	ribosomal protein L20 [N. tabacum]	73 ± 17
ACCT3-1	-	hypothetical protein [N. tabacum]	70 ± 30
ACCA3	+	mRNA C-7 [N. tabacum]	56 ± 26	**
ACGA3-1	+	No significant match	107 ± 16

^aTDF: transcript-derived fragment
^bExpression: up-regulated (+) or down-regulated (-) cDNA-AFLP signals detected in virus-infected leaves, compared to mock-infected leaves
^cCP: the coat protein accumulation levels of BaMV in knockdown plants
^dSignificance: Asterisks indicate statistically significant differences compared with the control plants (*p < 0.05, **p < 0.01, ***p < 0.001)

ISSN: 1471-2229