Skip to main content

Table 2 Transcript-derived fragments identified by cDNA-AFLP analysis and differentially expressed between Mock- and Bamboo mosaic virus-inoculated Nicotiana benthamiana plants.

From: Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamianaby cDNA-amplified fragment length polymorphism

TDFa

IDb

Length (bp)

Expressionc

Protein candidated

Ratioe

E valuef

Function: cell rescue, defense, death, and ageing:

   

ACTC1-1

AAM08661.1

207

-

putative disease resistance protein [Oryza sativa Japonica]

9/15

 

ACTC3-1

AAP03879.1

174

-

Avr9/Cf-9 rapidly elicited protein 216 [Nicotiana tabacum]

13/17

1e-05

ACTG3-1

AAB36652.1

213

+

immediate-early salicylate-induced [Nicotiana tabacum]

4/7

5e-19

ACCT8-1

AAC78594.1

129

+

Hcr2-2A [Solanum pimpinellifolium]

10/12

5e-04

ACCT10

AAA74119.1

121

+

SR1 Nt-rab7b [Nicotiana tabacum]

12/12

5e-04

ACAC6

CAA72515.1

205

+

heat shock protein [Arabidopsis thaliana]

7/7

1e-21

ACAC8-1

ACG31454.1

144

-

mpv17/PMP22 family protein [Zea mays]

5/10

 

ACTC8-1

ACG42715.1

120

-

mpv17/PMP22 family protein [Zea mays]

12/13

 

ACCT13

AAK11255.1

114

+

regulator of gene silencing [Nicotiana tabacum]

10/10

5e-04

ACGT10

CAD30209.1

140

+

putative auxin-induced protein 29 [Arabidopsis thaliana]

10/10

 

ACGT4

AAN63619.1

188

+

thioredoxin h-like protein [Nicotiana tabacum]

6/6

4e-09

ACCT14-1

AAC06242.1

102

-

late embryogenis abundant protein 5 [Nicotiana tabacum]

5/12

4e-03

ACCT12-1

ABH09088.1

116

-

putative membrane protein [Artemisia annua]

6/8

9e-14

ACGT11-1

CAA69901.1

118

+

plasma membrane polypeptide [Nicotiana tabacum]

8/10

1e-05

ACGA8-1

AAF24496.1

93

+

FH protein NFH1 [Nicotiana tabacum]

6/8

 

ACAG2-1

CAC81898.1

218

+

NEP1-interacting protein 2 [Arabidopsis thaliana]

9/10

5e-07

ACCA5-1

AAK40224.1

190

+

putative syntaxin of plants 52 [Oryza sativa Japonica]

8/10

1e-08

ACCA7-1

ABG73415.1

185

+

chloroplast pigment-binding protein CP29 [Nicotiana tabacum]

5/10

4e-05

ACCA8

ABD28323.2

183

+

excinuclease ABC, C subunit[Medicago truncatula]

8/8

4e-08

ACCA1-1

AAX95717.1

253

+

protease inhibitor/seed storage/LTP family [Oryza sativa Japonica]

5/10

 

ACCA10

AAM73656.1

168

+

AER [Nicotiana tabacum]

6/6

2e-22

ACAG4-1

AAD32145.1

176

-

Nt-iaa4.5 deduced protein [Nicotiana tabacum]

9/10

3e-05

Function: signal transduction:

   

ACCT1-1

CAX43672.1

322

-

CDK-activating kinase[Candida dubliniensis CD36]

6/9

 

ACCT7-1

BAF62637.1

135

+

DELLA protein [Phaseolus vulgaris]

10/12

4e-15

ACTG7-1

ABD34616.1

127

-

green ripe-like 1 [Solanum lycopersicum]

5/10

5e-04

ACCA2-1

AAS20952.1

204

+

calmodulin binding protein 25 [Arabidopsis thaliana]

6/10

5e-06

Function: transcription:

   

ACAC2

ABO42262.1

249

-

AT-hook DNA-binding protein [Gossypium hirsutum]

8/8

3e-04

ACGA4-1

AAL66977.1

141

+

putative cleavage and polyadenylation specificity factor [Arabidopsis thaliana]

8/10

 

ACCA6

AAM65499.1

186

+

AP2 domain transcription factor [Arabidopsis thaliana]

8/8

2e-05

ACAG11-1

BAC79914.1

57

-

homeobox transcription factor Hox7-like protein [Oryza sativa Japonica]

5/10

 

ACGA5

AAM14969.1

135

-

putative small nuclear ribonucleoprotein Prp4p [Arabidopsis thaliana]

7/7

 

ACCT4-1

AAB62807.1

220

+

S-adenosyl-methionine-sterol-C-methyltransferase [Nicotiana tabacum]

6/12

2e-34

ACGT1

AAK13103.1

285

-

helicase-like protein [Oryza sativa Japonica]

6/6

2e-03

ACGT5-1

AAA35148.1

183

+

transcription factor IIIB [Saccharomyces cerevisiae]

6/15

3e-05

Function: metabolism

   

ACCT5-1

ACG37370.1

193

-

lysine ketoglutarate reductaselysine trans-splicing [Zea mays]

7/12

2e-29

ACTG1-1

ACD13145.1

277

-

TOK1 potassium channel [Aspergillus fumigatus]

7/10

 

ACAC7-1

AAS46243.1

149

-

xyloglucan endotransglucosylase-hydrolase XTH7 [Solanum lycopersicum]

5/9

1e-13

ACCA11-1

ABN09771.1

139

-

glycosyl transferase, family 48 [Medicago truncatula]

10/16

1e-13

ACTC4-1

AAA34065.1

169

-

chloroplast carbonic anhydrase [Nicotiana tabacum]

10/18

6e-15

ACAG10

AAA34065.1

79

-

chloroplast carbonic anhydrase [Nicotiana tabacum]

10/10

1e-07

ACAG8

AAY17071.1

108

-

chloroplast carbonic anhydrase [Nicotiana benthamiana]

6/6

5e-21

ACGA10-1

BAA25639.1

76

-

NPCA1 [Nicotiana paniculata]

8/10

8e-04

Function: energy

   

ACGA9

AAD25541.

84

+

fructose-1,6-bisphosphatase precursor [Solanum tuberosum]

8/8

9e-08

ACGT2-1

CAA41713.1

193

-

photosystem II 23 kDa polypeptide [Nicotiana tabacum]

5/6

3e-20

ACGT3-1

AAA34053.1

190

+

beta-1,3-glucanase [Nicotiana tabacum]

5/6

2e-15

ACGT8-1

CAJ32461.1

160

+

putative chloroplast cysteine synthase 1 [Nicotiana tabacum]

10/15

1e-08

ACGT9-1

CAX42612.1

143

+

NADPH-dependent 1-acyl dihydroxyacetone [Candida dubliniensis CD36]

7/10

3e-23

ACAG5-1

AAM28014.1

158

+

granule-bound starch synthase [Peraphyllum ramosissimum]

7/16

1e-07

ACCA4

BAA28625.1

194

+

aldehyde oxidase [Arabidopsis thaliana]

10/10

2e-07

ACAG1

CAA74359.1

269

-

ferredoxin--NADP(+) reductase [Nicotiana tabacum]

10/10

7e-38

ACTC2

AAB39547.1

199

-

polygalacturonase isoenzyme 1 beta subunit [Solanum lycopersicum]

14/14

1e-28

ACCT2-1

AAC78441.1

295

-

12-oxophytodienoate reductase OPR2 [Arabidopsis thaliana]

7/8

2e-32

ACGT12

CAA44267.1

115

-

lipid transferase [Nicotiana tabacum]

8/8

6e-10

ACAG9

CAA44267.1

97

-

lipid transferase [Nicotiana tabacum]

10/10

7e-08

Function: translation

   

ACTC5-1

CAA77372.1

149

-

ribosomal protein L20 [Nicotiana tabacum]

6/17

2e-11

ACTG5-1

ABN08437.1

135

+

ribosomal protein L10 [Medicago truncatula]

7/10

9e-16

ACGA2-1

CAA77381.1

154

+

ribosomal protein S3 [Nicotiana tabacum]

10/17

1e-03

ACGT7-1

CAA77408.1

165

+

ribosomal protein L23 [Nicotiana tabacum]

6/10

2e-25

Function: unclassified

   

ACTC9-1

BAC98856.1

117

-

hypothetical protein [Brassica napus]

5/12

5e-06

ACTG9

AAW82556.

109

-

hypothetical protein [Phalaenopsis aphrodite subsp. formosana]

14/14

 

ACAC1-1

CAN77388.1

299

-

hypothetical protein [Vitis vinifera]

14/15

4e-41

ACAC5-1

CAN79807.1

240

-

unknown protein [Vitis vinifera]

8/10

8e-04

ACCT3-1

CAJ32479.1

270

-

hypothetical protein [Nicotiana tabacum]

5/10

1e-06

ACCT9-1

AAK20059.1

126

+

hypothetical protein [Oryza sativa Japonica]

6/10

 

ACGA1-1

ABW98323.1

174

+

hypothetical protein [Hemiselmis andersenii]

10/15

 

ACCA3

CAA45741.1

200

+

mRNA C-7 [Nicotiana tabacum]

8/8

6e-11

ACAG6

BAD46202.1

131

+

hypothetical protein [Oryza sativa Japonica]

8/8

 

ACCA9-1

AAM91702.1

174

+

unknown protein [Arabidopsis thaliana]

4/12

5e-14

No significant match

   

ACTG4

 

148

+

No significant match

8/8

 

ACTG6

 

134

-

No significant match

8/8

 

ACTG2-1

 

271

-

No significant match

5/12

 

ACAC3

 

246

-

No significant match

7/7

 

ACAC4-1

 

245

+

No significant match

4/7

 

ACTG8-1

 

117

+

No significant match

4/12

 

ACAC9-1

 

138

+

No significant match

5/8

 

ACAC11

 

118

-

No significant match

8/8

 

ACAC10-1

 

123

-

No significant match

7/12

 

ACCT6-1

 

151

+

No significant match

10/12

 

ACCT11-1

 

119

+

No significant match

10/12

 

ACGA3-1

 

151

+

No significant match

6/10

 

ACCT15-1

 

96

+

No significant match

6/12

 

ACTC6-1

 

135

+

No significant match

9/18

 

ACTC7-1

 

130

+

No significant match

6/12

 

ACGA6-1

 

107

-

No significant match

7/14

 

ACGA7

 

97

+

No significant match

8/8

 

ACGT6

 

181

-

No significant match

8/8

 

ACAG7-1

 

115

-

No significant match

5/8

 

ACAG3-1

 

186

+

No significant match

7/12

 

ACCA12-1

 

126

+

No significant match

10/14

 

ACAG12-1

 

47

+

No significant match

6/10

 
  1. aTDF: transcript-derived fragment
  2. bID: accession number of analogues identified by Tblastx
  3. cExpression: up-regulated (+) or down-regulated (-) cDNA-AFLP signals detected in virus-infected leaves, compared to mock-infected leaves dProtein candidate: Tblastx hit with the best E value
  4. eRatio: the number of the clone over the number of total clones sequenced
  5. fE value: only the value lower than 0.001 (1e-03) were shown according to statistic analysis with extremely significant hit