BaMV coat protein accumulation and the knockdown efficiency in each gene knockdown plants. The specific gene knockdown plants indicated below each statistic bar were inoculated with BaMV RNA. Protein and RNA extracts were analyzed for coat protein accumulation by Western blotting (A) and specific gene knockdown efficiency by semi-quantitative RT-PCR (B), respectively. The relative coat protein or RNA accumulation levels compared to that of control plants indicated as GFP (100%) were analyzed. The accumulation levels with standard deviations and their significances (t-test) of coat protein accumulation were shown above each statistic bar. Asterisks indicate statistically significant differences compared with the control indicated as GFP (*p < 0.01, **p < 0.05, ***p < 0.001).