The cDNA-AFLP profile in BaMV- and mock-transfected N. benthamiana leaves. RNA samples prepared from Mock- (M) and BaMV RNA-inoculated (I) leaves on 1, 3, 5, or 7 dpi were subjected to cDNA-AFLP analysis. Fluorescently labeled cDNA-AFLP fragments generated using the T-CA/M-GA primer pair were separated on a 6.5% polyacrylamide denaturing gel containing 8 M urea and imaged with a fluorescence scanner. The DNA size markers (bp) are indicated on the left side of the gel. The cDNA fragments detected at differential levels and eluted afterwards for further studies were marked with dash lines and designated as GA1 to 10 on the right side of the gel.