Figure 7
Analysis of the WRKY60:GUS reporter gene using protoplast transfection. A. Effects of ABA and W boxes on the WRKY60 promoter activity. Protoplasts from Col-0 wild type plants were transfected with the GUS reporter gene driven by the WRKY60 promoter (W60:GUS) or a mutant WRKY60 promoter in which the cluster of W-box sequences between -791 and -773 relative to the translation start codon were deleted (mW60:GUS). GUS activities were measured without or 12 h after the addition of 2 μM ABA. B. Effects of co-transfected WRKY18 and WRKY40 on the WRKY60 promoter activity. Protoplasts from wrky18/wrky40 double mutant plants were cotransfected with the W60:GUS or mW60:GUS reporter gene and an effect plasmid expressing WRKY18 (W18), or WRKY40 (W40) or two effector plasmids expressing the two WRKY proteins (W18+W40) driven by the WRKY60 promoter (W60:GUS). An empty effector plasmid was used as control. GUS activities were measured 12 h after co-transfection.