Figure 4

Induced expression of WRKY genes by ABA and abiotic stresses. A. Three-weeks-old wild-type plants were sprayed with water (Mock) or 5 μM ABA. Leaves from four treated plants were harvested at indicated time after the treatment for isolation of total RNA and analysis of transcripts using qRT-PCR. Expression level was defined as the ratio of qRT-PCR result of treated sample to its respective mock. The means and standard errors were calculated from three independent experiments. Asterisks mark statistically significant differences of expression level between ABA-treated-leaves harvested immediately and after indicated time. (Asterisks: p-value < 0.05; Double Asterisks: p-value < 0.01; by Student-Newman-Keuls Test). B. One-week-old wild-type seedlings were transferred to1/2 MS media without or with 150 mM NaCl or -0.75 MPa PEG. The seedlings were collected 24 hours after the transfer for total RNA isolation and analysis of transcripts using qRT-PCR. The means and standard errors were calculated from three independent experiments, all of which included no less than 20 seedlings per sample. Asterisks mark statistically significant differences of expression level between genotypically identical seedlings with or without indicated treatment. (Asterisks: p-value < 0.05; Double Asterisks: p-value < 0.01; by Student-Newman-Keuls Test).