Characterization of singlet oxygen-accumulating mutants isolated in a screen for altered oxidative stress response in Chlamydomonas reinhardtii

Table 1 Tolerance of gox mutants to various oxidative stress conditions

Mutant	HL	t-BOOH	NR	RB	MZ	MV
22D2	S	n	n	n	S	s
22D1	S	n	n	s	S	n
21E2	S	n	n	n	S	n
15B10	S	n	n	n	S	n
18C2	S	n	n	s	n	n
18F6	S	n	n	n	n	n
14H8	S	n	n	n	r	n
18G9	S	R	r	n	r	R
21B4	S	R	S	n	S	n
26D5	S	r	n	n	n	n
14A9	s	r	n	r	n	n
14B5	n	R	r	r	n	r
14C11	n	r	r	r	n	n
15H8	n	R	R	r	n	n
35H11	n	R	R	r	n	n
20H4	n	R	r	r	n	n
18B11	n	r	R	n	n	n
13D3	n	R	n	n	n	n
13H11	n	R	n	s	n	n
19H4	n	R	s	s	S	r

Abbreviations: HL: high light of 500 μmol photons m^-2 s^-1 PAR, t-BOOH: tert-butylhydroperoxide, NR: neutral red, RB: rose bengal, MZ: metronidazole, MV: methyl viologen. Tolerance was classified in five different categories compared to the wild-type strain: S: very sensitive, s: sensitive, n: no difference from wild-type, r: resistant, R: very resistant. Mutants could be divided into three different groups: HL sensitive mutants, t-BOOH resistant mutants and mutants with no or only minor changes in tolerance (not shown).

ISSN: 1471-2229