Figure 3

ABA and glucose sensitivity of AtERF13 overexpression lines. (A) Growth of AtERF13 OX lines. Three-week-old plants grown in soil. The numbers indicate line no. and the left panel shows the AtERF13 expression levels determined by Northern analysis. (B) Growth of the OX lines in the presence of 0.5 μM ABA. Seeds were germinated and grown for 10 days. (C) ABA dose response of shoot development measured by cotyledon greening efficiency. Seeds were germinated and grown for 11 days on MS medium containing various concentrations of ABA, and seedlings with green cotyledons were counted. Experiments were done in triplicates (n = 50 each), and the small bars indicate standard errors. (D) Root growth of the OX lines in the presence of 0.5 μM ABA. (E) Growth of the OX lines in the presence of 4% glucose. (F) Glucose dose response determined by cotyledon greening. Plants were grown on MS medium containing 3 or 4% glucose for 11 days before counting seedlings with green cotyledons. Experiments were conducted in triplicates (n = 50 each). The small bars represent standard errors.