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Figure 2 | BMC Plant Biology

Figure 2

From: Isolation and functional characterization of CE1 binding proteins

Figure 2

Expression patterns of CEBFs. (A) Induction patterns of CEBFs were determined by RT-PCR. UT, untreated plants. Plants were treated with 1/4MS, ABA, NaCl (Salt), 600 mM mannitol (Man) for 4 hrs, or placed at 4 C for 24 hr (Cold) before RNA was isolated. (B) Histochemical GUS staining of transgenic plants harboring AtERF13 promoter-GUS reporter gene construct. a. immature embryo. b. mature embryo. c, 5-day-old seedling. d, 15-day-old seedling. e, flower. f, immature silique. (C) Histochemical GUS staining of transgenic plants harboring the RAP2.4L promoter-GUS reporter gene construct. a. mature embryo. b. mature embryo. c, 2-day-old seedling. d, 5-day-old-seedling. e, 14-day-old seedling. f, roots of 14-day-old seedling. g, flower. h, mature silique. (D) Histochemical GUS staining of transgenic plants harboring RAP2.4 promoter-GUS reporter gene construct. a. mature embryo. b. mature embryo. c, 2-day-old seedling. d, 5-day-old-seedling. e, 14-day-old seedling. f, roots of 14-day-old seedling. g, flower. h, mature silique. In B-D, GUS staining was conducted for 20 hrs. (E) Subcellular localization of AtERF13, RAP2.4L and RAP2.4. Tobacco leaves were infiltrated with Agrobacterium as described in the Methods and observed with fluorescence microscope 40 hrs after infiltration. Bright field, fluorescence (YFP) and merged images of the tobacco leaves are shown.

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