Figure 6
The effect of the uncoupler FCCP (A) and protein synthesis inhibitor cycloheximide (B) on the CM-induced alamethicin resistance of tobacco cells. Resistance was measured as per cent of respiration rate remaining after 10 min incubation with 20 µg ml-1 alamethicin compared to the initial rate. Average of two independent experiments are shown with error bars representing SD. FCCP was added just before alamethicin addition, whereas cycloheximide was added before CM treatment (as described in Methods). The respiration increased 1.6 ± 0.1 and 1.7 ± 0.4 times in control and CM-treated cells, respectively, by the addition of FCCP, showing that respiration in the cell cultures became equally uncoupled from ATP synthesis.